fibronectin coating solution Search Results


fibronectin coating solution  (Axol Bioscience)
94
Axol Bioscience fibronectin coating solution
Fibronectin Coating Solution, supplied by Axol Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fibronectin coating solution/product/Axol Bioscience
Average 94 stars, based on 1 article reviews
fibronectin coating solution - by Bioz Stars, 2026-03
94/100 stars
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fibronectin coating solution  (Becton Dickinson)
90
Becton Dickinson fibronectin coating solution
Fibronectin Coating Solution, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fibronectin coating solution/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
fibronectin coating solution - by Bioz Stars, 2026-03
90/100 stars
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fibronectin coating solution  (Eppendorf AG)
90
Eppendorf AG fibronectin coating solution
Cryo-SEM images of seeding results for a simple <t>fibronectin</t> coat (Option 1) and a micropatterned coat (Option 2) (A) Option 1: Seeding on fibronectin-coated grids. The cells are spread randomly and a good portion of them is sitting on top of the grid bar, inaccessible to cryoET. Shown here are Adenovirus infected A549 cells on holey carbon coated 200 mesh (R2/1) gold Quantifoil finder grids frozen 47 h post seeding. (B) Option 2: Seeding on micropatterned grids. Nearly all squares in the center of the grid are occupied by cells growing in the middle of the grid square, ideally positioned for subsequent cryoFIB-milling and cryoET. Shown here are Vero E6 cells on an UltrAuFoil R 0.6/1 300-mesh grid frozen 6 h post seeding.
Fibronectin Coating Solution, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fibronectin coating solution/product/Eppendorf AG
Average 90 stars, based on 1 article reviews
fibronectin coating solution - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


Cryo-SEM images of seeding results for a simple fibronectin coat (Option 1) and a micropatterned coat (Option 2) (A) Option 1: Seeding on fibronectin-coated grids. The cells are spread randomly and a good portion of them is sitting on top of the grid bar, inaccessible to cryoET. Shown here are Adenovirus infected A549 cells on holey carbon coated 200 mesh (R2/1) gold Quantifoil finder grids frozen 47 h post seeding. (B) Option 2: Seeding on micropatterned grids. Nearly all squares in the center of the grid are occupied by cells growing in the middle of the grid square, ideally positioned for subsequent cryoFIB-milling and cryoET. Shown here are Vero E6 cells on an UltrAuFoil R 0.6/1 300-mesh grid frozen 6 h post seeding.

Journal: STAR Protocols

Article Title: Protocol for live-cell fluorescence-guided cryoFIB-milling and electron cryo-tomography of virus-infected cells

doi: 10.1016/j.xpro.2022.101696

Figure Lengend Snippet: Cryo-SEM images of seeding results for a simple fibronectin coat (Option 1) and a micropatterned coat (Option 2) (A) Option 1: Seeding on fibronectin-coated grids. The cells are spread randomly and a good portion of them is sitting on top of the grid bar, inaccessible to cryoET. Shown here are Adenovirus infected A549 cells on holey carbon coated 200 mesh (R2/1) gold Quantifoil finder grids frozen 47 h post seeding. (B) Option 2: Seeding on micropatterned grids. Nearly all squares in the center of the grid are occupied by cells growing in the middle of the grid square, ideally positioned for subsequent cryoFIB-milling and cryoET. Shown here are Vero E6 cells on an UltrAuFoil R 0.6/1 300-mesh grid frozen 6 h post seeding.

Article Snippet: Take the grids out of the fibronectin coating solution drop using your tweezer and thoroughly wash them by subsequently submerging them (10–15 times) in the prepared Eppendorf tubes filled with PBS.

Techniques: Infection

Option 1: Workflow seeding infected cells on grids 1. Apply fibronectin coating solution on glow-discharged holey carbon-coated gold finder grids (Quantifoil R2/1). 2. Let incubate for 1 h under UV-light. 3. Wet grids with PBS, wash in PBS and place in medium-filled ibidi chamber slide square. 4. Mix cells and virus at MOI:2 in Eppendorf tube. 5. Pipet small volume on grid, and check with light microscopy the cell distribution. Repeat until satisfied with the distribution.

Journal: STAR Protocols

Article Title: Protocol for live-cell fluorescence-guided cryoFIB-milling and electron cryo-tomography of virus-infected cells

doi: 10.1016/j.xpro.2022.101696

Figure Lengend Snippet: Option 1: Workflow seeding infected cells on grids 1. Apply fibronectin coating solution on glow-discharged holey carbon-coated gold finder grids (Quantifoil R2/1). 2. Let incubate for 1 h under UV-light. 3. Wet grids with PBS, wash in PBS and place in medium-filled ibidi chamber slide square. 4. Mix cells and virus at MOI:2 in Eppendorf tube. 5. Pipet small volume on grid, and check with light microscopy the cell distribution. Repeat until satisfied with the distribution.

Article Snippet: Take the grids out of the fibronectin coating solution drop using your tweezer and thoroughly wash them by subsequently submerging them (10–15 times) in the prepared Eppendorf tubes filled with PBS.

Techniques: Infection, Virus, Light Microscopy

Option 2. Micropatterning workflow 1. Preparation of passivated UltrAuFoil grids. 2. Photopatterning of passivated grids. 3. Application of fibronectin. 4. Seeding cells and washing-off any out of pattern cells.

Journal: STAR Protocols

Article Title: Protocol for live-cell fluorescence-guided cryoFIB-milling and electron cryo-tomography of virus-infected cells

doi: 10.1016/j.xpro.2022.101696

Figure Lengend Snippet: Option 2. Micropatterning workflow 1. Preparation of passivated UltrAuFoil grids. 2. Photopatterning of passivated grids. 3. Application of fibronectin. 4. Seeding cells and washing-off any out of pattern cells.

Article Snippet: Take the grids out of the fibronectin coating solution drop using your tweezer and thoroughly wash them by subsequently submerging them (10–15 times) in the prepared Eppendorf tubes filled with PBS.

Techniques:

Effect of a specific pattern on A549 cells growing on a micropatterned EM grid (A) Template of the pattern used for A549 cells. (B) Transmission light microscopy of A549 cells growing on an UltrAuFoil R0.6/1 300 mesh grid passivated with PLL-g-PEG with pattern A applied (dimension: 25 × 25 μm) and coated with 10 μg/mL fibronectin. The shape of pattern A is projected onto the grid square for visualization purposes.

Journal: STAR Protocols

Article Title: Protocol for live-cell fluorescence-guided cryoFIB-milling and electron cryo-tomography of virus-infected cells

doi: 10.1016/j.xpro.2022.101696

Figure Lengend Snippet: Effect of a specific pattern on A549 cells growing on a micropatterned EM grid (A) Template of the pattern used for A549 cells. (B) Transmission light microscopy of A549 cells growing on an UltrAuFoil R0.6/1 300 mesh grid passivated with PLL-g-PEG with pattern A applied (dimension: 25 × 25 μm) and coated with 10 μg/mL fibronectin. The shape of pattern A is projected onto the grid square for visualization purposes.

Article Snippet: Take the grids out of the fibronectin coating solution drop using your tweezer and thoroughly wash them by subsequently submerging them (10–15 times) in the prepared Eppendorf tubes filled with PBS.

Techniques: Transmission Assay, Light Microscopy

Journal: STAR Protocols

Article Title: Protocol for live-cell fluorescence-guided cryoFIB-milling and electron cryo-tomography of virus-infected cells

doi: 10.1016/j.xpro.2022.101696

Figure Lengend Snippet:

Article Snippet: Take the grids out of the fibronectin coating solution drop using your tweezer and thoroughly wash them by subsequently submerging them (10–15 times) in the prepared Eppendorf tubes filled with PBS.

Techniques: Virus, Recombinant, Modification, Saline, Clinical Proteomics, Software, Transferring, Injection, Electron Microscopy, Microscopy

Fibronectin Coating Solution

Journal: STAR Protocols

Article Title: Protocol for live-cell fluorescence-guided cryoFIB-milling and electron cryo-tomography of virus-infected cells

doi: 10.1016/j.xpro.2022.101696

Figure Lengend Snippet: Fibronectin Coating Solution

Article Snippet: Take the grids out of the fibronectin coating solution drop using your tweezer and thoroughly wash them by subsequently submerging them (10–15 times) in the prepared Eppendorf tubes filled with PBS.

Techniques: Concentration Assay